CHARACTERISTICS OF BULLS WITH DIFFERENT GENOTYPES OF THE OLR GENE ACCORDING TO THE PRODUCTIVITY OF FEMALE ANCESTORS
DOI 10.12737/2782-490X-2023-61-65 УДК 636.082:577.21
Keywords:
bull, PCR, DNA, genotype, gene OLR1, milk productionAbstract
The aim of the research was to assess the origin of Black-and-White breed bulls with different genotypes
according to the low-density lipoprotein receptor (OLR1) gene. The object of the study was one sample represented by
58 bulls-producers of black and motley breed belonging to JSC "Head breeding enterprise "Elite" of the Vysokogorsky
district of the Republic of Tatarstan. A preliminary assessment of the breeding value of Black-and-White bulls with differ
ent genotypes for one of the lipid metabolism marker genes (low-density lipoprotein receptor - OLR1), which are mainly
used in insemination of dairy cows bred in the Republic of Tatarstan, is presented. As a result of a molecular genetic study
(PCR-RFLP), the animal population was selected by groups, taking into account the genotype at the loci of the OLR1
gene. Evaluation and selection based on indicators of milk productivity of mothers, mothers and fathers of bull mothers
showed that producers with AA and AC genotypes of the low-density lipoprotein receptor gene had the greatest breeding
significance in terms of milk yield and mass fraction of fat in the milk of female ancestors. Thus, the parental index of
bulls with AA and AC genotypes of the OLR1 gene was 9071 kg, 3.90% and 9510 kg, 3.95% in terms of milk content and
fat mass, which is slightly higher than in bulls with CC genotype by 817 kg and 1256 kg (P<0.05) of milk, 0.03-0.08% fat,
respectively.
References
Anggraeni A. Genetic polymorphisms of the OLR1 and DGAT1 genes associated with milk components in
Holstein Friesian dairy cattle under an intensive management in Central Java // IOP Conference Series: Earth and
Environmental Science. 2019. V. 287. 012001. http://doi.org/10.1088/1755-1315/287/1/012001.
Шайдуллин Р. Р., Ганиев А. С. Оценка полиморфизма гена каппа-казеина у животных черно-пестрой
породы // Вестник Улья-новской государственной сельскохозяйственной академии. 2015. № 3 (31).
С. 104-109.
Rothschild M. F., Plastow G. S. Applications of genomics to improve livestock in the developing world //
Livest. Sci. 2014. V. 166. pp. 76-83. http://doi.org/10.1016/j.livsci.2014.03.020.
Quantitative trait loci mapping in dairy cattle: review and meta-analysis / M. S. Khatkar, P. C. Thomson, I.
Tammen, H. W. Raadsma // Genet. Sel. Evol. 2004. V. 36. pp. 163-190. http://doi.org/10.1186/1297-9686-36-2-
Atashi H., Asaadi A., Hostens M. Association between age at first calving and lactation performance, lactation curve, calving interval, calf birth weight, and dystocia in Holstein dairy cows // PLoS ONE. 2021. V. 16 (1):
e0244825. http://doi.org/10.1371/journal.pone.0244825.
Association of the OLR1 gene with milk composition in Holstein dairy cattle / H. Khatib, S. D. Leonard, V.
Schutzkus et al. // J. Dairy Sci. 2006. V. 89. pp. 1753-1760. http://doi.org/10.3168/jds.S0022-0302(06)72243-3.
Soltani-Ghombavani M., Ansari-Mahyari S., Edriss M. A. Association of a polymorphism in the 3' untranslated region of the OLR1 gene with milk fat and protein in dairy cows // Archiv Tierzucht 56. 2013. V. 32. pp. 328-
http://doi.org/10.7482/0003-9438-56-032.
Kowalewska-Luczak I., Czerniawska-Piatkowska E. Polymorphism in the OLR1 gene and functional traits of
dairy cattle // Veterinarski Arhiv. 2018. V. 88 (2). pp. 171-177. http://doi.org/10.24099/vet.arhiv.170228.
Mashhadi M. H. A research on association between SCD1 and OLR1 genes and milk production traits in
Iranian Holstein dairy cattle // Iranian J. of Applied Animal Sci. 2017. V. 7 (2). pp. 243-248.
Молочная продуктивность коров разных ветвей основных линий голштинской породы / А. И. Любимов, Е. Н. Мартынова, Е. В. Ачкасова и др. // Пермский аграрный вестник. 2021. № 2 (34). С. 69-76.
Генетические ресурсы бы-ков-производителей по ДНК-маркерам в Республике Татарстан / Р. Р.
Шайдуллин, Г. С. Шарафутдинов, Ф. С. Сибагатуллин, Ф. Р. Зарипов // Вестник Казанского государственного аграрного университета. 2016. Т. 11. № 4 (42). С. 60-68.
Молочная продуктивность коров, происходящих из перспективных ветвей голштинской породы / Р.
Р. Шайдуллин, Ч. А. Харисова, Т. М. Ахметов, А. С. Тенлибаева // Агробиотехнологии и цифровое земледелие. 2023. № 2 (6). С. 52-56.
Эффективность использования быков-производителей в Удмуртской Республике / Р. Р. Закирова, А.
П. Ямщиков, Г. Ю. Березкина, Ю. В. Исупова // Вестник Курской государственной сельскохозяйственной
академии. 2022. № 2. С. 109-113.
Русских Т. А., Бычкова В. А., Юдин В. М. Продуктивное долголетие коров чёрно-пёстрой и холмогорской пород // Пермский аграрный вестник. 2019. № 1 (25). С. 123-130.
Genetic potential of milk productivity of black and-white cows depending on selection and management / A.
I. Liubimov, E. N. Martynova, Yu. V. Isupova et al. // BIO Web of Conferences. International Scientific - Practical
Conference «Agriculture and Food Security: Technology, Innovation, Markets, Human Resources» (FIES 2019).
P. 158. http://doi.org/10.1051/bioconf/20201700158.
Продуктивные качества женских предков быков-производителей разных генотипов / Т. T. Тарчоков,
Х. М. Гасараева, М. Г. Тлейншева и др. // Вестник Курганской ГСХА. 2023. № 1 (45). С. 53-59.
Шайдуллин Р. Р., Шарафутдинов Г. С., Нигматуллин И. Н. Эффективные методы подбора в молочном скотоводстве / Практические рекомендации. Казань: Изд-во Казанского ГАУ, 2017. – 64 с.
Сравнительная характери-стика быков-производителей с разными ДНК-маркерами по молочной
продуктивно-сти женских предков / Ф. С. Сибагатуллин, Р. Р. Шайдуллин, Г. С. Шарафутди-нов и др. //
Ветеринарный врач. 2017. № 1. С. 52-59.
Komisarek J., Dorynek Z. Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls // J. Appl. Genet. 2009.
V. 50 (2). pp. 125-132.
Характеристика быков-производителей с комплексными генотипами генов BLG и INOS по молочной
продуктивности женских предков / Х. Х. Гильманов, С. В. Тюлькин, И. В. Ржанова, Р. Р. Вафин // Учёные
записки Казанской ГАВМ. 2020. Т. 241. № 1. С. 71-75. http://doi.org/10.31588/2413-4201-1883-241-1-71-76.
Оценка быков-производителей с разными генотипами генов соматотропинового каскада по молочной продуктивности и качеству молока ближайших женских предков / Л. Р. Загидуллин, И. Ю. Гилемханов,
Р. У. Зарипов и др. / Учёные записки Казанской ГАВМ. 2020. Т. 244. № 4. С. 86-92. http://
doi.org/10.31588/2413-4201-1883-244-4-86-92.
Evaluation towards stud bulls with different mixed genotypes relating to somatotropin cascade genes by
origin / L. Zagidullin, I. Gilemhanov, R. Khisamov, S. Tyulkin // BIO Web of Conferences. International scientificpractical conference “Agriculture and food security: Technology, innovation, markets, human resources” 2020. V.
00109. http://doi.org/10.1051/bioconf/20201700109.
Assessment on milk productivity and milk quality in cattle with different genotypes by HSP70.1 gene /
O. I. Efimova, L. R. Zagidullin, R. R. Khisamov et al. // IOP Conference Series: Earth and Environmental Science.
V. 604. 012016. http://doi.org/10.1088/1755-1315/604/1/012016.
Оценка по происхождению быков разных генотипов, ассоциированных с резистентностью к заболеваниям и качеством продукции / Х.Х. Гильманов, С.В. Тюлькин, Р.Р. Вафин, Н.С. Пряничникова // Молочное и мясное скотоводство. 2020. № 7. С. 29-33. http://doi.org/10.33943/MMS.2020.53.38.007.
Anggraeni A. Genetic polymorphisms of the OLR1 and DGAT1 genes associated with milk components in Holstein
Friesian dairy cattle under an intensive management in Central Java. IOP Conference Series: Earth and Environmental
Science. 2019. 287. 012001. http://doi.org/10.1088/1755-1315/287/1/012001.
Shaydullin R. R., Ganiyev A. S. [Evaluation of genetic polymorphism of kappa-casein gene in animals of blackmotley breed]. Vestnik Ul`yanovskoj gosudarstvennoj sel`skoxozyajstvennoj akademii. 2015; 3(31): 104-109. EDN:
UMIBGR.
Rothschild M. F., Plastow G. S. Applications of genomics to improve livestock in the developing world. Livest. Sci.
; 166: 76-83. http://doi.org/10.1016/j.livsci.2014.03.020.
Khatkar M. S., Thomson P. C., Tammen I. Quantitative trait loci mapping in dairy cattle: review and meta-analysis.
Genet. Sel. Evol. 2004; 36: 163-190. http://doi.org/10.1186/1297-9686-36-2-163.
Atashi H., Asaadi A., Hostens M. Association between age at first calving and lactation performance, lactation
curve, calving interval, calf birth weight, and dystocia in Holstein dairy cows. PLoS ONE. 2021; 16(1): e0244825. http://
doi.org/10.1371/journal.pone.0244825.
Khatib H., Leonard S. D., Schutzkus V. Association of the OLR1 gene with milk composition in Holstein dairy
cattle. J. Dairy Sci. 2006; 89: 1753-1760. http://doi.org/10.3168/jds.S0022-0302(06)72243-3.
Soltani-Ghombavani M., Ansari-Mahyari S., Edriss M. A. Association of a polymorphism in the 3' untranslated
region of the OLR1 gene with milk fat and protein in dairy cows. Archiv Tierzucht 56. 2013; 32: 328-334. http://
doi.org/10.7482/0003-9438-56-032.
Kowalewska-Luczak I., Czerniawska-Piatkowska E. Polymorphism in the OLR1 gene and functional traits of dairy
cattle. Veterinarski Arhiv. 2018; 88(2): 171-177. http://doi.org/10.24099/vet.arhiv.170228.
Mashhadi M. H. A research on association between SCD1 and OLR1 genes and milk production traits in Iranian
Holstein dairy cattle. Iranian J. of Applied Animal Sci. 2017; 7 (2): 243-248.
Martynova E. N., Yakimova V. Yu. [Milk yield and longevity of high yielding cows with different levels of
productivity depending on Holstein breed blood share]. Permskij agrarny`j vestnik. 2019; 2 (26): 128-136. EDN: BCSSIA.
Shaydullin R. R., Sharafutdinov G. S., Sibagatullin F. S. [Genetic resources of servicing bulls according to dna
markers in the republic of Tatarstan]. Vestnik Kazanskogo gosudarstvennogo agrarnogo universiteta. 2016; 11. 4(42): 60-
EDN: YPLNBP.
Shaydullin R. R., Kharisov Ch. A., Ahmetov T. M. [Dairy productivity of cows origining from perspective branches of the Holstanian breed]. Agrobiotexnologii i cifrovoe zemledelie. 2023; 2 (6): 52-56. EDN: ZJRYJS.
Zakirova R. R., Yamschikova A. P., Berezkina G. Yu. [Efficiency of use bulls in the Udmurt Republic]. Vestnik
Kurskoj gosudarstvennoj sel`skoxozyajstvennoj akademii. 2022; 2: 109-113. 14. Russkih T. A., Bychkova V. A., Yudin V. M. [Productive longevity of cows of Black pied and Kholmogorskaya
breeds]. Permskij agrarny`j vestnik. 2019; 1 (25): 123-130.
Liubimov A. I., Martynova E. N., Isupova Yu. V. Genetic potential of milk productivity of black and-white cows
depending on selection and management. BIO Web of Conferences. International Scientific - Practical Conference
«Agriculture and Food Security: Technology, Innovation, Markets, Human Resources» (FIES 2019). 2020: 158. http://
doi.org/10.1051/bioconf/20201700158.
Tarchokov T. T., Gasarayeva Kh. M., Tleynsheva M. G. [Productive qualities of female ancestors of bulls of different genotypes]. Vestnik Kurganskoj GSXA. 2023; 1 (45): 53-59.
Shaydullin R. R., Sharafutdinov G. S., Nigmatullin I. N. [Effective selection methods in dairy cattle breeding].
Prakticheskie rekomendacii. Kazan`: Izd-vo Kazanskogo GAU, 2017: 64.
Sibagatullin F. S., Shaydullin R. R., Sharafutdinov G. S. [The comparative characteristics of production bulls with
various dna-markers on dairy efficiency of female ancestors]. Veterinarny`j vrach. 2017; 1: 52-59.
Komisarek J., Dorynek Z. Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated
breeding values for functional and production traits in Polish Holstein-Friesian bulls. J. Appl. Genet. 2009; 50 (2):
-132.
Gilmanov Kh. Kh., Tyulkin S. V., Rzhanova I. V. [Characteristics of bull-producers with complex genotypes
genes of BLG and iNOS by milk productivity of female ancestors]. Uchyony`e zapiski Kazanskoj GAVM. 2020; 241. 1:
-75. http://doi.org/10.31588/2413-4201-1883-241-1-71-76.
Zagidullin L. R., Gilemhanov I. Yu., Zaripov R. U. [Evaluation of breeding bulls with different genotypes of somatotropin cascade genes for milk productivity and milk quality of the closest female ancestors]. Uchyony`e zapiski Kazanskoj GAVM. 2020; 244. 4: 86-92. http://doi.org/10.31588/2413-4201-1883-244-4-86-92.
Zagidullin L., Gilemhanov I., Khisamov R. Evaluation towards stud bulls with different mixed genotypes relating
to somatotropin cascade genes by origin. BIO Web of Conferences. International scientific-practical conference
“Agriculture and food security: Technology, innovation, markets, human resources” 2020; 17: 00109. http://
doi.org/10.1051/bioconf/20201700109.
Efimova O. I., Zagidullin L. R., Khisamov R. R. Assessment on milk productivity and milk quality in cattle with
different genotypes by HSP70.1 gene. IOP Conference Series: Earth and Environmental Science. 2020; 604: 012016.
http://doi.org/10.1088/1755-1315/604/1/012016.
Gilmanov Kh. Kh., Tyulkin S. V., Vafin R. R. [Assessment by origin of bulls of different genotypes associated
with disease resistance and product quality]. Molochnoe i myasnoe skotovodstvo. 2020; 7: 29-33. http://doi.org/10.33943/
MMS.2020.53.38.007.
