DNA IDENTIFICATION OF SPECIES OF MEAT AND PLANT RAW MATERIALS IN FINISHED PRODUCTS BASED ON PCR
DOI 10.12737/2782-490X-2025-69-80 УДК 577.29
Keywords:
real-time polymerase chain reaction, species-specific DNA, species identification, meat, fish productsAbstract
The aim of this work is to identify the species of raw materials in meat and fish products using real-time
polymerase chain reaction (RT-PCR). This method is capable of detecting with high accuracy the presence of the declared
species-specific fragment contained in the sample in very small quantities. The studies were conducted in 2024 at Tatar
branch of Federal Center for Animal Health. (FSBI “VNIIZZh”). For comprehensive laboratory studies, 18 types of meat
and fish products sold in retail food stores located in the area of responsibility of FSBI “VNIIZZh” were selected. As a
result of RT-PCR, it was found that in such products as meatballs, “Aristokrat” rump steak, “Afanasevskie” cutlets, triangle with beef, the presence of a sufficient amount of species-specific chicken DNA characteristic of chicken meat was not
detected, which confirms that the type of meat (except poultry) corresponds to the declared one. The products bavette with
turkey and mushrooms, turkey in pepper sauce and basmati rice with basil, khinkali made of Halal beef, dumplings “Beef”,
pink salmon fillet correspond to the declared type of meat and fish. However, pig DNA was identified in the boiled sausages “With beef”, and pollock DNA was found in the cod liver, which indicates falsification and discrepancy with the declared composition of the product. When examining dumplings, jellied meat, sausages, boiled sausage for the presence of
soy ingredients, soy DNA was not detected in these products. Therefore, one of the most accurate methods capable of
identifying the species of products and detecting falsification is the polymerase chain reaction.
For citation: Shaydullin R.R., Tyulkin S.V., Gilemkhanov I.Yu., Valiullina E.F., Sadriev A.R. DNA identification of
species of meat and plant raw materials in finished products based on PCR. Agrobiotechnologies and digital farming.
2025. 1 (13). 69-80 p.
References
Литература
Identification of meat origin in food products – a review / Sh. Rahmati, N. M. Julkapli, W. J. Basirun, W.
Yehye // Food Control. 2016. Vol. 68. P. 379–390. https://doi.org/10.1016/j.foodcont.2016.04.013
Lysine Supply Is a Critical Factor in Achieving Sustainable Global Protein Economy / I. Leinonen, P. P. M.
Iannetta, B. M. Rees [et al.] // Frontiers in Sustainable Food Systems. 2019. Vol. 3. Article 27. https://
doi.org/10.3389/fsufs.2019.00027
A 20-year analysis of reported food fraud in the global beef supply chain / K. Robson, M. Dean, S. Brooks, S.
Haughey, C. Elliott // Food Control. 2020. Vol. 116. Р. 107310. https://doi.org/10.1016/j.foodcont.2020.107310
Complementary molecular methods detect undeclared species in sausage products at retail markets in Canada / A. M. Naaum, Shu Chen, H. Shehata [et al.] // Food Control. 2018. Vol. 84. P. 339-344. https://
doi.org/10.1016/j.foodcont.2017.07.040
Серегин И. Г., Никитченко Д. В., Михеева. М. И. Совершенствование ветсанэкспертизы икры лососевых рыб // Вестник Российского университета дружбы народов. Серия: Агрономия и животноводство. 2017.
Т. 12. № 3. С. 279–288. https://doi.org/10.22363/2312-797X-2017-12-3-279-288
Калюжная Т. В., Орлова Д. А., Родак Г. Н. Идентификация икры лососевых пород рыб с помощью
полимеразной цепной реакции с наблюдением в реальном времени // Международный вестник ветеринарии.
4. 88-92. https://doi.org/10.52419/issn2072-2419.2021.4.88
Калюжная Т. В., Орлова Д. А., Родак Г. Н. Ветеринарно-санитарная экспертиза икорных продуктов //
Вопросы нормативно-правового регулирования в ветеринарии. 2021. № 2. С. 133-136. https://
doi.org/10.17238/issn2072-6023.2021.2.133.
Simple and fast multiplex PCR method for detection of species origin in meat products / M. Izadpanah, N.
Mohebali, Z. E. Gorji [et al.] // Journal of Food Science and Technology. 2017. Vol. 55(2). P. 698–703. https://
doi.org/10.1007/s13197-017-2980-2
Молочная продуктивность и качество молока коров с разными генотипами olr1 и линейной принадлежностью / М. Ламара, Л. Р. Загидуллин, Т. М. Ахметов [и др.] // Ученые записки Казанской государственной академии ветеринарной медицины им. Н.Э. Баумана. 2023. Т. 253. № 1. С. 163-167. https://
doi.org/10.31588/2413_4201_1883_1_253_163
Межлинейный полиморфизм гена каппа-казеина и его влияние на молочную продуктивность коров /
Р. Р. Шайдуллин, Г. С. Шарафутдинов, А. Б. Москвичёва, Б. Г. Зиганшин, С. В. Тюлькин // Достижения
науки и техники АПК. 2019. Т. 33. № 5. С. 51-54. https://doi.org/10.24411/0235-2451-2019-10512
Identification of meat species by TaqMan-based real-time PCR assay / Z. Kesmen,
A. Gulluce, F. Sahin, H. Yetim // Meat Science. 2009. Vol. 82(4). P. 444-449. https://doi.org/10.1016/
j.meatsci.2009.02.019
Коновалова Е. Н., Гладырь Е. А., Зиновьева Н. А. Видовая идентификация мяса птицы в животноводческой продукции с применением метода полимеразной цепной реакции // Достижения науки и техники
АПК. 2011. № 10. С. 67-69.
Выявление ДНК курицы в мясной продукции, реализуемой в Москве и Московской области методом
полимеразной цепной реакции / З. Н. Меньшикова, К. О. Любкина, З. С. Девришова [и др.] // Ветеринария
Кубани. 2020. № 5. С. 26-29 https://doi.org/ 10.33861/2071-8020-2020-5-26-29
Галкин А., Трепалина Е. Анализ примесей свинины в мясном сырье и готовой продукции методом
ПЦР // Мясной ряд. 2019. № 2(76). С. 34.
ДНК‑идентификация животных для выявления фальсификаций продуктов питания / М. Михайлова,
Р. Шейко, Е. Лагун [и др.] // Наука и инновации. 2020. №10. С. 40–45. https://doi.org/10.29235/1818-9857-
-10-40-45.
Минаев М. Ю., Лисицын Б. А., Фомина Т. А. Методы ПЦР для определения сырьевых компонентов в
готовой продукции // Мясная индустрия. 2008. № 6. С. 36-37.
Direct pentaplex PCR assay: an adjunct panel for meat species identification in Asian food products / P.
Thanakiatkrai, J. Dechnakarin, R. Ngasaman, T. Kitpipit // Food Chemistry. 2019. Vol. 271. P. 767–772. https://
doi.org/10.1016/j.foodchem.2018.07.143
Karabagias I. K. Advances of spectrometric techniques in food analysis and food authentication implemented with chemometrics // Foods. 2020. Vol. 9(11). Р. 1550. https://doi.org/10.3390/foods9111550
Современные методы выявления фальсификации сырья и пищевой продукции, детекции несанкционированных примесей в кормах / А. М. Кривецкая, Г. В. Мозгова, Н. И. Дробот, В. С. Остапчик, А. Н. Островская // Endless light in science. 2024. №1. Р. 26-32. https://doi.org/10.24412/2709-1201-2024-3-5
Минаев М. Ю., Солодовникова Г. И., Курбаков К. А. Выбор ДНК матрицы для обоснования порогового уровня технически неустранимых примесей мяса птицы в готовой мясной продукции // Теория и практика переработки мяса. 2017. Т. 2. №1. Р. 27-36. https://doi.org/10.21323/2414-438X-2017-2-1-27-36
Цифровая капельная ПЦР - высокотехнологичный подход для идентификации и определения абсолютного количества ДНК-мишеней / Н. И. Дробот, А. Н. Островская, В. С. Остапчик., Г. В. Мозгова // Сучаснi тенденцiї розвитку освiти й науки: проблеми та перспективи: сборник научных работ. 2020. Вып. 6. С.
-352.
Комарова И. Н., Серегин И. Г., Валихов А. Ф. Полимеразная цепная реакция – современный метод
выявления фальсификации мясного сырья и продуктов // Мясная индустрия. 2004. №2. С. 37–41.
Yayla M. E. A., Ekinci Doğan C. Development of a new and sensitive method for the detection of pork
adulteration in gelatin and other highly processed food products // Food Additives & Contaminants: Part A. 2021. Т.
№. 6. С. 881-891. https://doi.org/10.1080/19440049.2021.1902574
References
Rahmati Sh, Julkapli NM, Basirun WJ, Yehye W. Identification of meat origin in food products – a review. Food
Control. 2016; Vol.68. 379-390 p. https://doi.org/10.1016/j.foodcont.2016.04.013
Leinonen I, Iannetta PPM, Rees BM. Lysine supply is a critical factor in achieving sustainable global protein economy. Frontiers in Sustainable Food Systems. 2019; Vol.3. Article 27. https://doi.org/10.3389/fsufs.2019.00027
Robson K, Dean M, Brooks S, Haughey S, Elliott C. A 20-year analysis of reported food fraud in the global beef
supply chain. Food Control. 2020; Vol.116. 107310 p. https://doi.org/10.1016/j.foodcont.2020.107310
Naaum AM, Chen Shu, Shehata H. Complementary molecular methods detect undeclared species in sausage products at retail markets in Canada. Food Control. 2018; Vol.84. 339-344 p. https://doi.org/10.1016/j.foodcont.2017.07.040
Seregin IG, Nikitchenko DV, Mikheeva MI. [Improving the veterinary sanitary examination of salmon caviar].
Vestnik Rossiyskogo universiteta druzhby narodov. Seriya: Agronomiya i zhivotnovodstvo. 2017; Vol.12. 3. 279-288 p.
https://doi.org/10.22363/2312-797X-2017-12-3-279-288
Kalyuzhnaya TV, Orlova DA, Rodak GN. [Identification of salmon caviar using real-time polymerase chain reaction]. Mezhdunarodnyy vestnik veterinarii. 2021; 4. 88-92 p. https://doi.org/10.52419/issn2072-2419.2021.4.88
Kalyuzhnaya TV, Orlova DA, Rodak GN. [Veterinary and sanitary examination of caviar products]. Voprosy normativno-pravovogo regulirovaniya v veterinarii. 2021; 2. 133-136 p. https://doi.org/10.17238/issn2072-6023.2021.2.133.
Izadpanah M, Mohebali N, Gorji ZE. Simple and fast multiplex PCR method for detection of species origin in meat
products. Journal of Food Science and Technology. 2017; Vol.55(2). 698-703 p. doi.org/10.1007/s13197-017-2980-2
Lamara M, Zagidullin LR, Akhmetov TM. [Milk productivity and milk quality of cows with different olr1 genotypes and lineage]. Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy meditsiny im. N.E. Baumana.
; Vol.253. 1. 163-167 p. https://doi.org/10.31588/2413_4201_1883_1_253_163
Shaydullin RR, Sharafutdinov GS, Moskvicheva AB, Ziganshin BG, Tyulkin SV. [Interline polymorphism of the
kappa-casein gene and its effect on milk productivity of cows]. Dostizheniya nauki i tekhniki APK. 2019; Vol.33. 5. 51-54
p. https://doi.org/10.24411/0235-2451-2019-10512
Kesmen Z, Gulluce A, Sahin F, Yetim H. Identification of meat species by TaqMan-based real-time PCR assay.
Meat Science. 2009; Vol.82(4). 444-449 p. https://doi.org/10.1016/j.meatsci.2009.02.019
Konovalova EN, Gladyr EA, Zinoveva NA. [Species identification of poultry meat in livestock products using the
polymerase chain reaction method]. Dostizheniya nauki i tekhniki APK. 2011; 10. 67-69 p.
Menshikova ZN, Lyubkina KO, Devrishova ZS. [Detection of chicken DNA in meat products sold in Moscow and
Moscow region using the polymerase chain reaction method]. Veterinariya Kubani. 2020; 5. 26-29 p. https://doi.org/
33861/2071-8020-2020-5-26-29
Galkin A, Trepalina E. [Analysis of pork impurities in raw meat and finished products by PCR]. Myasnoy ryad.
; 2(76). 34 p.
Mikhaylova M, Sheyko R, Lagun E. [DNA identification of animals to detect food falsifications]. Nauka i innovatsii. 2020; 10. 40-45 p. https://doi.org/10.29235/1818-9857-2020-10-40-45.
Minaev MYu, Lisitsyn BA, Fomina TA. [PCR methods for determining raw material components in finished products]. Myasnaya industriya. 2008; 6. 36-37 p.
Thanakiatkrai P, Dechnakarin J, Ngasaman R, Kitpipit T. Direct pentaplex PCR assay: an adjunct panel for meat
species identification in Asian food products. Food Chemistry. 2019; Vol.271. 767-772 p. https://doi.org/10.1016/
j.foodchem.2018.07.143
Karabagias IK. Advances of spectrometric techniques in food analysis and food authentication implemented with
chemometrics. Foods. 2020; Vol.9(11). 1550 p. https://doi.org/10.3390/foods9111550
Krivetskaya AM, Mozgova GV, Drobot NI, Ostapchik VS, Ostrovskaya AN. [Modern detecting methods for falsification of raw materials and food products, detection of unauthorized impurities in feed]. Endless light in science. 2024; 1.
-32 p. https://doi.org/10.24412/2709-1201-2024-3-5
Minaev MYu, Solodovnikova GI, Kurbakov KA. [Selection of DNA matrix to substantiate the threshold level of
technically irremovable impurities of poultry meat in finished meat products]. Teoriya i praktika pererabotki myasa. 2017;
Vol.2. 1. 27-36 p. https://doi.org/10.21323/2414-438X-2017-2-1-27-36
Drobot NI, Ostrovskaya AN, Ostapchik VS, Mozgova GV. [Digital droplet of PCR - a high-tech approach for
identification and determination of the absolute amount of target DNA]. Suchasni tendentsiї rozvitku osviti i nauki:
problemi ta perspektivi: sbornik nauchnykh rabot. 2020; issue 6. 347-352 p.
Komarova IN, Seregin IG, Valikhov AF. [Polymerase chain reaction - a modern method for detecting falsification
of meat raw materials and products]. Myasnaya industriya. 2004; 2. 37-41 p.
Yayla MEA, Ekinci Dogan C. Development of a new and sensitive method for the detection of pork adulteration in
gelatin and other highly processed food products. Food Additives & Contaminants: Part A. 2021; Vol.38. 6. 881-891 p.
